SP2B - MelAutim

Role of T cells during checkpoint-controlled autoim-munity and immune-therapy

The overall goal of this subproject is to detect and understand molecular pathways that trigger inflammation and autoimmunity in response to cancer immunotherapies such as immune checkpoint inhibitors. We plan to determine the contribution of T cells (CD4 and CD8) to the anti-tumor immune response and autoimmune-triggered inflammation, respectively. To this end, we will generate multicolour flow-cytometry data to dissect T cells sub-sets present during checkpoint therapy-induced autoimmunity and anti-tumour immune response. Using network analysis, the data will be used in SP4 and SP5 to determine computationally molecular pathways and cell-to-cell interaction mechanisms pivotal in the activation of T cells during checkpoint therapy-induced autoimmunity and anti-tumour immune response, insights that later will be experimentally validated in this subproject through in vitro experiments and mouse models.